Crop wild relative populations of Beta vulgaris allow direct mapping of agronomically important genes

Autor/in: Capistrano-Gossmann, G. G.; D. Ries, D. Holtgräwe, A. Minoche, T. Kraft, S. L. M. Frerichmann, T. Rosleff Soerensen, J. C. Dohm, I. González, M. Schilhabel, M. Varrelmann, H. Tschoep, H. Uphoff, K. Schütze, D. Borchardt, O. Toerjek, W. Mechelke, J. C. Lein, A W. Schechert, L. Frese, H. Himmelbauer, B. Weisshaar, F. J. Kopisch-Obuch
Jahr: 2017
Zeitschrift: Nature Communications
Seite/n: DOI: 10.1038/ncomms15708

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Abstract

Rapid identiﬁcation of agronomically important genes is of pivotal interest for crop breeding. One source of such genes are crop wild relative (CWR) populations. Here we used a CWR population of o200 wild beets (B. vulgaris ssp. maritima), sampled in their natural habitat, to identify the sugar beet (Beta vulgaris ssp. vulgaris) resistance gene Rz2 with a modiﬁed version of mapping-by-sequencing (MBS). For that, we generated a draft genome sequence of the wild beet. Our results show the importance of preserving CWR in situ and demonstrate the great potential of CWR for rapid discovery of causal genes relevant for crop improvement. The candidate gene for Rz2 was identiﬁed by MBS and subsequently corroborated via RNA interference (RNAi). Rz2 encodes a CC-NB-LRR protein. Access to the DNA sequence of Rz2 opens the path to improvement of resistance towards rhizomania not only by marker-assisted breeding but also by genome editing.

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