Characterisation of effectors of the pathogen Cercospora beticola in sugar beet
Start:
1 December 2025 at the earliest
Support:
PD Dr. Sebastian Liebe
Contact:
liebe@ifz-goettingen.deQuestion:
The fungus Cercospora beticola is the pathogen responsible for Cercospora leaf spot disease, which mainly affects sugar beet. This is one of the most important diseases in sugar beet cultivation. In the course of co-evolution, the fungal pathogen has specialised in sugar beet. So-called effectors are of great importance for the pathogenicity of the pathogen. These are proteins that are released into the host organism. There, the effectors suppress the immune defence or induce cell death, for example. In the course of a genetic population analysis, two effectors were identified that could be important for the pathogenicity of the pathogen. The aim of this Master's thesis is to characterise these two effectors in more detail. First, the sequence variability in different Cercospora populations will be analysed. Gene expression will be measured in vitro and in planta in plant and laboratory experiments. In addition, transient expression of both effectors will be performed in the experimental host Nicothiana benthamiana. Various molecular biology techniques will be learned as part of the work (e.g. cloning, gene expression measurement with qPCR). Extensive laboratory and greenhouse experiments are also part of the work.